By collaborating with University of California, San Diego, the research team led by Xia Lanqin at Institute of Crop Sciences of CAAS, established a novel CRISPR/Cas mediated allele replacement system in crop plants by using DNA and/or RNA transcripts as repair templates and successfully achieved precise gene replacement of rice ALS gene. The newly established gene replacement system overcomes the difficulties in delivering sufficient donor repair template for targeted gene replacement in plant. This research was published in Nature Biotechnology.
RNA transcript-templated homology-directed DNA repair of double-stranded DNA breaks